2010年毕业于河北大学生物工程专业获得学士学位；
2015年获得中国农业科学院生物化学与分子生物学专业理学博士学位；
2015年07月至2018年06月，中国农业科学院生物技术研究所博士后（获评优秀博士后）；
2018年07月至2020年12月，中国农业科学院生物技术研究所助理研究员；
2021年1月至今，中国农业科学院生物技术研究所副研究员。
人才团队
刘兴健博士、副研究员
liuxingjian@caas.cn
研究中心
农业微生物学研究中心
创新团队
微生物蛋白设计与智造创新团队
课 题 组
张志芳课题组
招生方向
微生物学
学习工作经历
主要研究领域与成果
主要从事昆虫杆状病毒表达系统在动物健康养殖领域应用的相关研究，探索昆虫杆状病毒表达系统的优化和改造，解析动物用细胞因子和酶类在动物体内的作用机制，创制动物健康养殖用蛋白制剂。
近年主持国家自然科学基金项目1项，主持国家重点实验室开放课题2项，参与国家自然科学基金、国家重点研发计划、转基因重大专项、“973”和“863”等多项课题研究。发表SCI研究论文17篇，其中第一（含并列第一）或通讯作者论文8篇。
主要论著
1. Liu, XJ; Wei, YL; Li, YN; Li, HY; Yang, X; Yi, YZ; Zhang, ZF, A Highly Efficient and Simple Construction Strategy for Producing Recombinant Baculovirus Bombyx mori Nucleopolyhedrovirus, PLoS One. 2016 Mar 23;11(3):e0152140.
2. Liu, XJ#; Li, YN#; Hu, XY; Yi, YZ*; Zhang, ZF*, Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector, ONCOTARGET. 2017, 8 (62): 106017-106025.
3. Liu, XJ; Yang, X; Mehboob, A; Hu, XY; Yi, YZ; Li, YN*; Zhang, ZF*, A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons, MICROBIOLOGYOPEN. 2020, 9 (3): e979.
4. Li, YN#; Liu, XJ#; Tang, P; Zhang, H; Qin, QL; Zhang, ZF, Genome sequence and organization of the Mythimna (formerly Pseudaletia) unipuncta granulovirus Hawaiian strain, SCIENTIFIC REPORTS. 2021, 11 (1): 414.
5. Li, YN; Jiang, F; Shi, XF; Liu, XJ; Yang, HP; Zhang, ZZ, Identification and Characterization of the Cyclin-Dependent Kinases Gene Family in Silkworm, Bombyx mori, DNA AND CELL BIOLOGY, 2015, 35(1), Pp. 1–11.
6. Zheng, XX; Wang, SK; Zhang, WJ; Liu, XJ; Yi, YZ; Yang, ST; Xia, XZ; Li, YN; Zhang, ZF, Development of a VLP-based vaccine in silkworm pupae against rabbit hemorrhagic disease virus. Int Immunopharmacol. 2016 Sep 3;40:164-169.
7. Arslan, M; Yang, X; Santhakumar, D; Liu, XJ; Hu, XY; Munir M; Li, YN; Zhang, ZF, Dynamic Expression of Interferon Lambda Regulated Genes in Primary Fibroblasts and Immune Organs of the Chicken. Genes (Basel). 2019 Feb 14;10(2). pii: E145.
8. Zhao, LL; Du, MT; Liu, XJ; Zhang, ZD; Zhang, ZF; Meng, XL; Li, YN, Interaction with the Receptor SLAM and Baculovirus Surface Display of Peste des petits ruminants Virus Hemagglutinin. DNA Cell Biol. 2020 Jun;39(6):992-999.
9. Yang, X; Arslan M; Liu, XJ; Song, HZ; Du, MT; Li, YZ; Zhang, ZF, IFN-γ establishes interferon-stimulated gene-mediated antiviral state against Newcastle disease virus in chicken fibroblasts. Acta Biochim Biophys Sin (Shanghai). 2020 Mar 18;52(3):268-280.
10. Yi, YZ; Li JL; Zong, ZP; Liu, XJ; Song, HZ; Wang, HN; Zhang, ZF; Zhang, H; Li, YN, Cloning, expression, and characteristic analysis of the novel β-galactosidase from silkworm, Bombyx mori. Genesis. 2021 Sep;59(9):e23446.
11. Zhao, LL#; Liu, XJ#; Tang, K; Zhang, ZF; Zhang, H*; Li, YN*. A Renewed Appreciation of Helicoverpa armigera Nucleopolyhedrovirus BJ (Formerly Helicoverpa assulta Nucleopolyhedrovirus) with Whole Genome Sequencing. Viruses. 2022 Mar 16;14(3):618. doi: 10.3390/v14030618. PMID: 35337025; PMCID: PMC8951894.
12. Song, HZ; Liu, XJ; Gao, XT; Li, JL; Shang, YT; Gao, WS; Li, YN; Zhang, ZF*, Transcriptome analysis of pre-immune state induced by interferon gamma inhibiting the replication of H9N2 avian influenza viruses in chicken embryo fibroblasts. Infect Genet Evol. 2022 Jul 7:105332. doi: 10.1016/j.meegid.2022.105332. Epub ahead of print. PMID: 35811034.
13. Li, D; Song, HZ; Li, JL; Liu, XJ; Gao, XT; Wu, T; Zhang, ZF*; Li, YN*, Expression and Evaluation of a Novel PPRV Nanoparticle Antigen Based on Ferritin Self-Assembling Technology. Pharmaceutics. 2022; 14(9):1902.
14. Liu, XJ#; Song, HZ#; Jiang, JM; Gao, XT; Yi, YZ; Shang, YT; Li, JL; Li, D; Zeng, Z; Li, YN*; Zhang, ZF*, Baculovirus-expressed self-assembling SARS-CoV-2 nanoparticle vaccines targeting the S protein induce protective immunity in mice. Process Biochemistry. 2023, 129, 200–208.
15. Li, JL#; Gao, XT#; Liu, XJ; Wu, T; Song, HZ; Gao, WS; Jia, H; Li, YN*; Zhang, ZF*, The host transcriptome change involved in the inhibitory effect of exogenous interferon-γ on Getah virus replication. Front Microbiol. 2023 Jun 28;14:1214281.
16. Gao, XT#; Li, JL#; Wu, T; Dou, JP; Zhang, WR; Jia, H; Zhang, ZF; Liu, XJ*; Li, YN*, The Isolation and Characterization of a Novel Group III-Classified Getah Virus from a Commercial Modified Live Vaccine against PRRSV, Viruses, 2023, 15(10)：2090.
17. Zhang, WR#; Wang, HN#; Wu, T; Gao, XT; Shang, YT; Zhang, ZF; Liu, XJ*; Li, YN*; A SARS-CoV-2 nanobody displayed on the surface of human ferritin with high neutralization activity, International Journal of Nanomedicine, 2024, 19: 2429-2440.
近年主持国家自然科学基金项目1项，主持国家重点实验室开放课题2项，参与国家自然科学基金、国家重点研发计划、转基因重大专项、“973”和“863”等多项课题研究。发表SCI研究论文17篇，其中第一（含并列第一）或通讯作者论文8篇。
主要论著
1. Liu, XJ; Wei, YL; Li, YN; Li, HY; Yang, X; Yi, YZ; Zhang, ZF, A Highly Efficient and Simple Construction Strategy for Producing Recombinant Baculovirus Bombyx mori Nucleopolyhedrovirus, PLoS One. 2016 Mar 23;11(3):e0152140.
2. Liu, XJ#; Li, YN#; Hu, XY; Yi, YZ*; Zhang, ZF*, Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector, ONCOTARGET. 2017, 8 (62): 106017-106025.
3. Liu, XJ; Yang, X; Mehboob, A; Hu, XY; Yi, YZ; Li, YN*; Zhang, ZF*, A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons, MICROBIOLOGYOPEN. 2020, 9 (3): e979.
4. Li, YN#; Liu, XJ#; Tang, P; Zhang, H; Qin, QL; Zhang, ZF, Genome sequence and organization of the Mythimna (formerly Pseudaletia) unipuncta granulovirus Hawaiian strain, SCIENTIFIC REPORTS. 2021, 11 (1): 414.
5. Li, YN; Jiang, F; Shi, XF; Liu, XJ; Yang, HP; Zhang, ZZ, Identification and Characterization of the Cyclin-Dependent Kinases Gene Family in Silkworm, Bombyx mori, DNA AND CELL BIOLOGY, 2015, 35(1), Pp. 1–11.
6. Zheng, XX; Wang, SK; Zhang, WJ; Liu, XJ; Yi, YZ; Yang, ST; Xia, XZ; Li, YN; Zhang, ZF, Development of a VLP-based vaccine in silkworm pupae against rabbit hemorrhagic disease virus. Int Immunopharmacol. 2016 Sep 3;40:164-169.
7. Arslan, M; Yang, X; Santhakumar, D; Liu, XJ; Hu, XY; Munir M; Li, YN; Zhang, ZF, Dynamic Expression of Interferon Lambda Regulated Genes in Primary Fibroblasts and Immune Organs of the Chicken. Genes (Basel). 2019 Feb 14;10(2). pii: E145.
8. Zhao, LL; Du, MT; Liu, XJ; Zhang, ZD; Zhang, ZF; Meng, XL; Li, YN, Interaction with the Receptor SLAM and Baculovirus Surface Display of Peste des petits ruminants Virus Hemagglutinin. DNA Cell Biol. 2020 Jun;39(6):992-999.
9. Yang, X; Arslan M; Liu, XJ; Song, HZ; Du, MT; Li, YZ; Zhang, ZF, IFN-γ establishes interferon-stimulated gene-mediated antiviral state against Newcastle disease virus in chicken fibroblasts. Acta Biochim Biophys Sin (Shanghai). 2020 Mar 18;52(3):268-280.
10. Yi, YZ; Li JL; Zong, ZP; Liu, XJ; Song, HZ; Wang, HN; Zhang, ZF; Zhang, H; Li, YN, Cloning, expression, and characteristic analysis of the novel β-galactosidase from silkworm, Bombyx mori. Genesis. 2021 Sep;59(9):e23446.
11. Zhao, LL#; Liu, XJ#; Tang, K; Zhang, ZF; Zhang, H*; Li, YN*. A Renewed Appreciation of Helicoverpa armigera Nucleopolyhedrovirus BJ (Formerly Helicoverpa assulta Nucleopolyhedrovirus) with Whole Genome Sequencing. Viruses. 2022 Mar 16;14(3):618. doi: 10.3390/v14030618. PMID: 35337025; PMCID: PMC8951894.
12. Song, HZ; Liu, XJ; Gao, XT; Li, JL; Shang, YT; Gao, WS; Li, YN; Zhang, ZF*, Transcriptome analysis of pre-immune state induced by interferon gamma inhibiting the replication of H9N2 avian influenza viruses in chicken embryo fibroblasts. Infect Genet Evol. 2022 Jul 7:105332. doi: 10.1016/j.meegid.2022.105332. Epub ahead of print. PMID: 35811034.
13. Li, D; Song, HZ; Li, JL; Liu, XJ; Gao, XT; Wu, T; Zhang, ZF*; Li, YN*, Expression and Evaluation of a Novel PPRV Nanoparticle Antigen Based on Ferritin Self-Assembling Technology. Pharmaceutics. 2022; 14(9):1902.
14. Liu, XJ#; Song, HZ#; Jiang, JM; Gao, XT; Yi, YZ; Shang, YT; Li, JL; Li, D; Zeng, Z; Li, YN*; Zhang, ZF*, Baculovirus-expressed self-assembling SARS-CoV-2 nanoparticle vaccines targeting the S protein induce protective immunity in mice. Process Biochemistry. 2023, 129, 200–208.
15. Li, JL#; Gao, XT#; Liu, XJ; Wu, T; Song, HZ; Gao, WS; Jia, H; Li, YN*; Zhang, ZF*, The host transcriptome change involved in the inhibitory effect of exogenous interferon-γ on Getah virus replication. Front Microbiol. 2023 Jun 28;14:1214281.
16. Gao, XT#; Li, JL#; Wu, T; Dou, JP; Zhang, WR; Jia, H; Zhang, ZF; Liu, XJ*; Li, YN*, The Isolation and Characterization of a Novel Group III-Classified Getah Virus from a Commercial Modified Live Vaccine against PRRSV, Viruses, 2023, 15(10)：2090.
17. Zhang, WR#; Wang, HN#; Wu, T; Gao, XT; Shang, YT; Zhang, ZF; Liu, XJ*; Li, YN*; A SARS-CoV-2 nanobody displayed on the surface of human ferritin with high neutralization activity, International Journal of Nanomedicine, 2024, 19: 2429-2440.